How-To·5 min read·January 15, 2025
How to Reconstitute Peptides Safely
A step-by-step guide to mixing lyophilized peptides with bacteriostatic water — without ruining a $200 vial.
What you'll need
- Vial of lyophilized (freeze-dried) peptide
- Bacteriostatic water (BAC water) — NOT regular saline
- Insulin syringe (typically 1mL / 100 unit U-100)
- Alcohol swabs
Step-by-step
- Wipe both vials (peptide + BAC water) with an alcohol swab.
- Draw your BAC water — the amount depends on how concentrated you want each dose. Use our reconstitution calculator to figure out exactly how much.
- Inject slowly down the side of the vial. Don't blast it directly onto the powder — peptides are fragile.
- Swirl gently. Never shake. Shaking can denature the peptide.
- Wait 30 seconds. The powder should fully dissolve into a clear solution.
- Refrigerate — most reconstituted peptides last 30 days at 2-8°C.
Common mistakes
- Using tap water or sterile water (no preservative = bacteria growth)
- Shaking the vial violently
- Storing at room temp after mixing
- Forgetting to label with the reconstitution date